Journal: PloS one
Article Title: Kinetic analysis of mouse brain proteome alterations following Chikungunya virus infection before and after appearance of clinical symptoms.
doi: 10.1371/journal.pone.0091397
Figure Lengend Snippet: Figure 2. 2D-DIGE analysis (pH 3–10) of mock (M)-, early (E), late paralytic (LP) and late tetanus-like (LT) CHIKV-infected brain samples. (A) Representative data from a 2D-DIGE experiment using a 10% SDS-polyacrylamide gel with the pH 3–10 range is shown. Proteins from M-, E- and LP- and LT- CHIKV-infected brain samples were labeled with Cy5 or Cy3 cyanine dyes, as described in Table S1. As determined by Progenesis SameSpot software, protein spots that were differentially regulated between the four experimental groups (|FC| $1.3 and p #0.05), were submitted to mass spectrometry for identification. The numbers annotated on the gel corresponded to master gel numbers of deregulated protein spots. Spots were all identified as Mus musculus proteins and were listed in Table 1. Spots differentially modified between E and mock- (B), LP- and E- (C), LT and E- (D), LP and M- (E) and LT and M- (F) infected samples are represented by red (up-regulated) or blue (down-regulated) dots. doi:10.1371/journal.pone.0091397.g002
Article Snippet: Images were cropped with ImageQuant software (GE Healthcare UK) and further analyzed using the software package Progenesis SameSpot v2 software (Nonlinear Dynamics, Newcastle upon Tyne, UK).
Techniques: Infection, Labeling, Software, Mass Spectrometry, Modification